Species-specific quantification of probiotic lactobacilli in yoghurt by quantitative real-time PCR
نویسندگان
چکیده
منابع مشابه
Quantification of total bacteria, enterobacteria and lactobacilli populations in pig digesta by real-time PCR.
Jejunum digesta samples were taken from weaning pigs in order to evaluate real-time PCR (qPCR) as a method for quantifying pig gut bacteria. Total bacteria, lactobacilli and enterobacteria were quantified by qPCR and the results were compared with those obtained with traditional methods: 4',6-diamidino-2-phenylindole (DAPI staining) for total bacteria, selective culture for lactobacilli and ent...
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Identification of genes with invariant levels of gene expression is a prerequisite for validating transcriptomic changes accompanying development. Ideally expression of these genes should be independent of the morphogenetic process or environmental condition.We report here the validation of internal control gene i.e.TPS (trehalose 6-phosphate-synthase) in cotton (Gossypium spp), using TaqMan sy...
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Unlike classical end-point analysis PCR, real-time PCR provides the data required for quantification of the target nucleic acid. The results can be expressed in absolute terms by reference to external quantified standards or in relative terms compared to another target sequence present within the sample. Absolute quantification requires that the efficiency of the amplification reaction is the s...
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ژورنال
عنوان ژورنال: Journal of Applied Microbiology
سال: 2013
ISSN: 1364-5072
DOI: 10.1111/jam.12341